Part:BBa_K1761005:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Application TU Eindhoven 2015

LargeBit is used as an intracellulair signaling domain. In combination with SmallBit (part BBa_K1761006 [1]) a Bioluminescence signal can be generated by adding furimazine. We verified that this part alone with furimazine gives no signal. The same goes for SmallBit. Therefore, these parts are ideally as signaling components if you want to check if two parts are present.

Application TU Eindhoven 2016

iGEM TU Eindhoven used this part in combination with SmallBiT ("https://parts.igem.org/Part:BBa_K1761006"). These parts were linked to the CT52 protein. These linked parts are:
CT52-SmallBiT"https://parts.igem.org/Part:BBa_K2065000" and CT52-LargeBiT"https://parts.igem.org/Part:BBa_K2065007"
Those two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. This system was used as a read out method for T14-3-3 heterodimers. It is schemtically visualised in figure 1. For more detailed information about the application the NanoBiT system visit our wiki "http://2016.igem.org/Team:TU-Eindhoven/Read-out".

Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52

Application UCNZ 2024

We used this part in combination with LgBiT (BBa_K1761005) linked to the cage forming protein Encapsulin (creating BBa_K5330020 and BBa_K5330021). These parts act as a reporter system for cage formation (see figure below and the respective pages for the parts mentioned above).

User Reviews

UNIQ6f2f6a8093a1c019-partinfo-00000001-QINU UNIQ6f2f6a8093a1c019-partinfo-00000002-QINU